Analysis of Population Structure of Magnaporthe grisea Using Genome Specific Microsatellite Markers

نویسندگان

  • K. Madhan Mohan
  • M. Sheshu Madhav
  • M. Srinivas Prasad
  • S. J. S. Rama Devi
  • G. Ram Kumar
  • B. C. Viraktamath
چکیده

In order to understand the pathogen’s genetic diversity and dynamics of fungal populations, 34 rice blast isolates collected from various blast endemic areas of India were analyzed using Magnaporthe grisea genome specific microsatellite markers (MGM) and repeat element based Pot2 primer. All the blast isolates showed average pair wise similarities in the range of 0.15 to 0.9 and suggested the large variations with in the isolates collected from the different places. To know the genetic relationship among the blast isolates, a dendogram was generated based on analysis of MGM and Pot2 primers separately and in combination using SHAN/ UPGMA program. The cluster analysis grouped all the blast isolates in to different clusters mostly based on the location, from where they were collected. Based on International blast differential lines containing single resistance genes, it was identified that AVR Pi-k gene was present predominantly in isolate collected from Nellore in costal Andhra Pradesh; and AVR Pi-2 and AVR Pi-4 genes in isolates collected from Mandya (Karnataka). The isolates collected from Almora, Ranchi and Nawagam showed the presence of AVR Pi-1 and AVR Pi-4a genes. The present study helped us to understand the population diversity and their AVR genes spectra in the blast hotspot regions of India, which can be useful in deployment strategies for blast resistance genes in rice improvement programmes.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Polymorphism of Microsatellite Sequence within ABC Transporter Genes in Phytopathogenic Fungus, Magnaporthe Grisea

Thirteen polyporphic microsatellite markers suitable for population genetic structure analysis and ABC transporter and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations and rice varieties. Preliminary results indicated that each locu...

متن کامل

Polymorphism of Microsatellite Sequence within Protein Kinase ORFs in Phytopathogenic Fungus, Magnaporthe Grisea

Eighteen polymorphic microsatellite markers suitable for population genetic studies and protein kinase encoding genic variation measurement were developed for rice blast fungus Magnaporthe grisea. Polymorphism was evaluated by using 46 isolates collected from diverse geographical locations and rice varieties. Preliminary results indicate that each locus harbors two to

متن کامل

Characterization and Phylogenetic Analysis of Magnaporthe spp. strains on Various Hosts in Iran

Background: Populations of Magnaporthe, the causal agent of rice blast disease, are pathotypically and genetically diverse and therefore their interaction with different rice cultivars and also antagonistic microorganisms are very complicated. Objectives: The objectives of the present study were to characterize phylogenetic relationships of 114 native  Magnaporthe strains, isolated from rice a...

متن کامل

Association Analysis for Important Quantitative and Morphological Traits in Cultivars and Advanced Lines of Soybean (Glycine max (L.)) using Microsatellite Markers

IExtended Abstract Introduction and Objective: The economic value of a genotype depends on its various traits and therefore the accurate knowledge of genetic behavior and identification of genomic locus involved in controlling these traits can help the breeder to improve genotypes. Material and Methods: In this study, the relationship between microsatellite markers with some important agrono...

متن کامل

Population structure and dynamics of Magnaporthe grisea in the Indian Himalayas.

The population genetics of Magnaporthe grisea, the rice blast pathogen, were analyzed in a center of rice diversity (the Uttar Pradesh hills of the Indian Himalayas) using multilocus and single-, or low-copy, DNA markers. Based on DNA fingerprinting with the multilocus probe MGR586 and single-locus probes, 157 haplotypes clustered into 56 lineages (at >/=70% MGR586 band similarity, each with un...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2012